Viability of Escherichia coli cells under long-term cultivation in a rich nutrient medium.

Rich Medium Composition Affects Escherichia coli Survival, Glycation, and Mutation Frequency during Long-Term Batch Culture.

Bacteria such as Escherichia coli are frequently grown to high density to produce biomolecules for study in the laboratory. To achieve this, cells can be incubated in extremely rich media that increase overall cell yield. In these various media, bacteria may have different metabolic profiles, leading to changes in the amounts of toxic metabolites produced. We have previously shown that stresses...

Long-term cultivation and differentiation of human erythroleukemia cells in a protein-free chemically defined medium.

To examine whether a human erythroleukemia cell line, K-562, can proliferate and differentiate without protein supplements, long-term cultivation of the cells was carried out in a protein-free chemically defined medium. By the use of stepwise decreases in the fetal bovine serum concentration, continuous growth of K-562 cells was established in a protein-free F-10 medium. The cells have been pro...

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

Adaptation of Escherichia coli to Long-Term Serial Passage in Complex Medium: Evidence of Parallel Evolution

Experimental evolution of bacterial populations in the laboratory has led to identification of several themes, including parallel evolution of populations adapting to carbon starvation, heat stress, and pH stress. However, most of these experiments study growth in defined and/or constant environments. We hypothesized that while there would likely continue to be parallelism in more complex and c...

Studies of Nutrient Media for Tissue Cells in Vitro II. An Improved Protein-flee Chemically for Long-Term Cultivation of Strain Defined Medium L-929 Cells

F u n d a m e n t a l p r o b l e m s of g r o w t h a n d t h e sc reen ing of c h e m o t h e r a p e u t i c a g e n t s b e c o m e m o r e n e a r l y de f inab le in precise c h e m i c a l t e r m s w h e n q u a n t i t a t i v e p rocedu re s (5, 9) for e s t i m a t ing p ro l i f e r a t i on are used in t h e p resence of a c h e m i c a l l y def ined b a c k g r o u n d m e d i u ...